NI LUH GDE SUMARDANI. Viability and
Fertility of Spermatozoa in Modified BTS and Zorlesco Extender Stored in
Different Places for Swine Artificial Insemination Program. Under the direction
of TUTY L. YUSUF and POLLUNG H. SIAGIAN.
The optimal storage temperature for preserve
boar semen is 17 - 18 °C. The temperature fluctuations can decrease sperm
viability. The aim of this study was to obtain a boar semen extender for
Artificial Insemination (AI) at certain distance area. The observation on
viability and fertility of spermatozoa in Modified Beltsville Thawing Solution
(M-BTS) and Zorlesco (M-Zorlesco) extender in different storage, and the effect
of storage system i.e : room temperature, refrigerator, and styrofoam box were
conducted for this purpose. Semen from three years old Yorkshire boar (n = 3)
were collected twice a week by hand method. Semen characteristics and their
quality were evaluated macro and microscopically. These semen were added with
M-BTS and M-Zorlesco extender up to fourfold volume (ratio 1 : 3). This is base
on the assumption of AI dose of 2 - 3 x 109cells/80mL. All samples were divided
into three tube and stored at room temperature (22 °C), styrofoam box (18 °C)
and refrigerator (15 °C), and their motility and viability were evaluated every
six hours for 42 hours observation.
The results showed that fresh semen
characteristics were good, with sperm motility of 65.56 ± 3.91% and viability
of 87.70 ± 6.34 %. The best extender found in this experiment of 42 hours
observation was M-Zorlesco extender with average sperm motility of 54.76 ±
12.76% in styrofoam box and 54.17 ± 12.60% in refrigerator. On the other hand,
their average of sperm motility in BTS (control) of 49.37 ± 17.37% in styrofoam
box and 50.19 ± 17.36 % in refrigerator were slightly lower than that of the
former one, but statistically it is not significant. The semen diluted in
M-Zorlesco extender stored for 42 hours in styrofoam box that showed the best
result. This show that quality of boar’s sepermatozoa can be maintained at 15 -
18 oC. Conception rate with semen diluted in M-Zorlesco and M-BTS extenders
were high (83.33%).
In conclusion, M-Zorlesco extender can
maintain the quality of spermatozoa stored in styrofoam box and refrigerator
for about 42 hours with sperm motility 40 - 50%, and the styrofoam box can be
used as an alternatif container for insemination program in the field.
Keywords: Viability, fertility,
extender, storage, boar sperm
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