M DENNY SAPTO PRASETYO.
Effectivity of Liquid Organic Acid to Inactivate Newcastle Disease. .Supervised
by SRI MURTINI and RETNO DAMAYANTI SOEJODONO
The objective of this study
was to determine the ability of organic acid liquid to inactivate Newcastle Disease
Virus (NDV). The first study was toxicity of organic acid in embryonated
chicken because the second study were used embryonated chicken eggs to evaluate
the capability of organic acid to inactivate NDV. Toxicity of organic acid was
conducted by inoculated organic acid liquid concentration 0%, 0.2%, 0.3% and
0.4% in 5 eggs/concentration in allantoic cavity. The variable of the
observation of toxicity test was the mortality 5 and embryo body weight. The
ability test of organic acid to inactivated NDV was conducted in 50 embryonated
eggs. The eggs were divided into 5 groups with each groups contains 10 eggs as
much as 2mL. NDV titre 105 EID50/mL were incubated with organic acid 0%, 0.2%,
0.3%, and 0,4% equal volume in room temperature (27 ºC) for 30 minutes. Each
suspension were inoculated in the 10 embryonated eggs and incubated for 2-7
days. At days two 5 embryonated eggs from each concentration of organic acid
which still live were chilled at 4°C and then observed the present of virus,
patological changed, and embryo weight. The 5 eggs from each group were
incubated until days 7. On day 7 all eggs were chilled at 4°C and then observed
the growth of virus, patological changed, and embryo weight. The result showed
that organic acid concentration 0.2%, 0.3%, 0.4% inactivated 50%, 100%, and 90%
NDV. The conclusion of this research was organic acid liquid at the
concentration 0.3% effective to inactivate NDV in vitro.
Keyword : Embryo Chicken,
Inactivated Virus, Organic Acid, Newcastle Disease, Toxicity
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