Identification of the
Lactoferrin (LTF EcoRI) Gene Polymorphism in HolsteinFriesian Cattle in BIB
Lembang, BBIB Singosari and BET Cipelang
Mumpunie, G. E. , C. Sumantri,
and A. Anggraeni
Lactoferrin (LTF) is a gene
controlling milk protein component in which having the characteristic as
antimicrobial. LTF contained in the milk has function to prevent diarhea,
whereas the function of LTF for the cows is to prevent mastitis in the udder.
By looking at the role of the LTF gene, an attempt of improving LTF component
in the milk is necessary through the selection process conducted at the DNA
level. This study was aimed to identify the polymorphism of the LTF EcoRI gene
in Holstein-Friesian (HF) cattle. The LTF EcoRI gene polymorphism was
identified with Polymerase Chain Reaction-Restricsion Fragment Length
Polymorphism (PCR-RFLP) method using EcoRI enzyme. Genotyping was perfomed in
89 HF cattle from Balai Inseminasi Buatan (BIB) Lembang (17 heads), Balai Besar
Inseminasi Buatan (BBIB) Singosari (32 heads) and Balai Embrio Ternak (BET)
Cipelang (40 heads). Genotyping was also conducted in four breeds of beef
cattle as a comparison for Limosin (14 heads), Angus (5 heads), Simental (13
heads) and Brahman (5 heads) from BET Cipelang.
Results from the analysis of
the allele frequencies of the LTF EcoRI gene in HF cattle in three locations
and beef cattle showed that the A allele had a higher frequency than the B
allele. The total frequencies of the A and B allels in HF cattle were 0,77 and
0,23 respectively. Genotypes for HF cattle observed were found for AA and AB,
whereas the BB genotype was not found. The genotype frequencies of all cattle
(HF and beef cattle) had the same proportion, genotype AA and AB had the same
value (0,5), whereas the genotype BB was 0. Heterozygosity (Ho) value of LTF
EcoRI gene were higher than those of heterozygosity value for all cattle. The
conclusion was that LTF EcoRI gene in HF and beef cattle observed was
polymorphic.
Keywords: Genetic
polymorphism, Holstein-Friesian, Lactoferrin gene, PCR-RFLP
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