Antibacterial Activity of N-hexane Extract of Attacus atlas Imago againsts Staphylococcus aureus and Escherichia coli

Posted by Sekilas Peternakan on

Tuesday, 9 January 2018

MUHAMAD ALIF NA. Antibacterial Activity of N-hexane Extract of Attacus atlas Imago againsts Staphylococcus aureus and Escherichia coli. Supervised by USAMAH AFIFF and DAMIANA RITA EKASTUTI.

Antibacterial was a substance that can be used to inhibit the growth of bacterias or to kill them. Antibacterial could be taken directly from the nature or could be produced in semisintetic form or modificated from biosintetic molecules. The aim of the research was to find out the potency of antibacterial activity of n-hexane extract of Attacus atlas imago againsts Staphylococcus aureus and Escherichia coli.. The extraction of sample was done by maseration method using incubator shaker for 72 hours in 37 temperature with 125 rpm speed. Then the filtrat was evaporated using rotary evaporator in 60 temperature and 0.045 mPa pressure during 27 minutes. Test of antibacterial activity was done by using well difusion method and compared by positive control (antibiotic ciprofloxacin) and negative control (n-hexane). The result showed the inhibitory zone of extract with the diameter of 6.00 mm on isolate of Staphylococcus aureus and Escherichia coli. Based on the result of the test known that n-hexane extract of Attacus atlas imago has no antibacterial activity on Staphylococcus aureus and Escherichia coli.

Key words: antibacterial, Attacus atlas, extract, imago, n-hexane

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