Detection of Anti-KHV DNA Vaccine Transmission toward Bacteria in Common Carp Cultivation Media

Posted by Sekilas Peternakan on

Wednesday 23 August 2017

DINDA JANUARI CIPTA. Detection of Anti-KHV DNA Vaccine Transmission toward Bacteria in Common Carp Cultivation Media. Supervised by SRINURYATI and ALIMUDDIN.

KHV is an infectious disease that attacks common carp in various stadia and causing 80-100% of death. One of ways to prevent KHV infection is by using DNA vaccine. DNA vaccine is a genetically modified product that was feared to be transmitted to bacteria and affects genetic diversity. This research was performed to detect of anti-KHV DNA vaccine transmission into bacteria of common carp rearing water. Vaccination was conducted by dipping and oral vaccination methods. Bacteria from dipping vaccination and aquaria were isolated on 6 hours, 4 th day, and 7 th day post vaccination while bacteria from pond were isolated on 6 hours, 4 th day and 7 th day after fish were reared in pond.

Bacteria as many as 50 µL were spread in triptic soy agar containing ampicilline (50 µg/µL). Bacteria that grow in the TSA contain ampisilin were 103 bacteria and the amount of bacteria that identified to species level by using API kit were 11 bacteria; 2 colonies were identified as Aeromonas hydrophila, 4 colonies were identified as Bacillus cereus, 2 colonies were identified as Bacillus subtilis, 2 colonies were identified as Staphylococcus auricularis dan 1 colony was identified as Enterobacter sakazakii. The existence of DNA vaccine in the bacteria was tested by using cracking and PCR methods. The results of cracking indicated the absence of bacteria containing anti-KHV DNA vaccine. Analysis of bacteria by PCR method showed that the bacteria could not uptake anti-KHV DNA vaccine from the environment, so the conclusion was anti-KHV DNA vaccine did not transmitted to bacteria in common carp rearing water.

Keywords: KHV, DNA vaccine, transmission, bacteria in the water, common carp.

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