M. ALRAFKI. The Viability Immunoglobulin G Anti Escherichia coli originated from microencapsulated bovine colostrum. Supervised by SRI MURTINI and ANITA ESFANDIARI.
Colostral immunoglobulin G has potential for developed because has contains substances resistant to specific pathogens. The objective of this experiment was to evaluate the viability of microencapsulated IgG anti Escherichia coli during storage for 8 weeks at 4 oC. The microcapsules were made with the different ratio between IgG and alginate i.e. 20:80 (P1), 30:70 (P2), 40:60 (P3) respectively. The microcapsules were stored at 4 oC and observed for 8 weeks. The IgG consentration were analyzed using indirect Enzyme Linked Immunosorbent Assay (ELISA). Results of the experiment indicated that no significant changes on the concentration of IgG on P1 and P2 microcapsules (p >0.05) up to 4 weeks of storage, while changes were documented on P3 microcapsules at week 4 of storage. In conclusion, microcapsules with the ratio of IgG colostrums and alginate 20:80 and 30:70 better if compare to of those with ratio 40:60 stored within 4 weeks at 4 oC.
Keywords: alginate, bovine colostrum, chitosan, IgG, microcapsules.
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